In this study we further analyzed the epidemiology of mcmv and corn lethal necrosis disease clnd in china. Electrostatic properties of cowpea chlorotic mottle virus and. Mcmv was first described in maize from peru in 1974 castillo and hebert, 1974 and thereafter was reported on maize plants in the united states and. Molecular characterization of a novel sunflower chlorotic. Therefore, visual detection of maize chlorotic mottle virus is a potentially useful tool for middle or smallscale corporations and entryexit inspection and quarantine bureaux to detect maize. The clcmv genome is 3,880 nt in length with five open reading frames orfs encoding a 27kda protein orf 1, an 87kda replicase protein orf 2, two. Maize lethal necrosis disease mln is an emerging disease in east africa caused by the introduction of maize chlorotic mottle virus mcmv. Pdf visual detection of maize chlorotic mottle virus using. If you desire to recieve a personal copy with price information. Our objective was to gain insights into the genetic architecture underlying the resistance to mlnd by genomewide association. Pdf maize lethal necrosis disease mlnd develops from synergistic co infection by sugarcane mosaic virus and maize chlorotic. Detection and charac terization of maize chlorotic mottle virus. The complete nucleotide sequence of pepper mottle virus genomic rna. Maize chlorotic mottle virus is the third plant virus responsible for initial spread of the in kansas since.
The concentration of maize chlorotic mottle virus mcmv was up to 5. The complete nucleotide sequence 4436 nt of a yunnan. Based on previous editions produced by cimmyt maize. Maize chlorotic dwarf of maize is caused by maize chlorotic dwarf virus mcdv. Maize chlorotic mottle virus mcmv is a positivesense singlestranded rna virus, and the sole member of the machlomovirus genus in the tombusviridae family. Sunflower chlorotic mottle virus sucmov is the most wide. As a mixed infection with wheat streak mosaic virus wsmv, sugarcane mosaic virus, or maize dwarf mosaic virus, it induces a synergism referred to as corn lethal necrosis. Mcmv is one of the most important plant pathogenic viruses and can induce various symptoms such as mild mosaic and lead crop losses of 10 to 15% in natural field infections and losses of up to 59% in experimental maize plots 3. Maize chlorotic mottle virus exhibits low divergence. One virus is maize chlorotic mottle virus mcmv, which when combined with another virus sugarcane mosaic virus scmv results in the development of mln disease, leading to serious plant damage. Maize chlorotic mottle virus mcmv is the only identified member of the genus machlomovirus in the family tombusviridae king et al.
Maize chlorotic mottle machlomovirus and wheat streak mosaic. The virus infects members of the family gramineae sugarcane, maize. Maize chlorotic mottle machlomovirus and wheat streak mosaic rymovirus concentrations increase in the synergistic disease. Wft to transmit mcmv, imported maize seeds from thailand were germinated in an insect. Detection and characterization of maize chlorotic mottle virus.
Maize lethal necrosis agricultural research council. New research on maize lethal necrosis disease in kenya the. Reporting service articles about maize chlorotic mottle virus mcmv00 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Agdia negative control for maize chlorotic mottle virus mcmv. Pdf detection and characterization of maize chlorotic mottle virus. Maize chlorotic mottle virus mcmv was first reported in maize in china in 2009. The virus occurs in south central nebraska, north central kansas, and south america.
First report of maize chlorotic mottle virus infecting. Analysis of gene functions in maize chlorotic mottle virus. Maize chlorotic mottle virus is a plant pathogenic virus. Maize chlorotic mottle virus mcmv is not widespread in the united states.
It remains a challenge to detect this virus to prevent its introduction, infection and its transmission throughout fields. Laboratory transmission of maize chlorotic mottle virus by. The disease causes symptoms ranging from leaf tissue mottling and malformed ears to premature plant death. The viri ons of this singlestranded rna virus are isometric, and the singlecomponent particles have a smooth spherical or hexagonal shape scheets 2010. Maize chlorotic mottle virus mcmv 1 is one type of virus from the genus machlomovirus and was first found in maize from peru in 1973. Plant research to atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. Mcmv can cause corn lethal necrosis disease clnd resulting from synergistic interaction between this virus and maize dwarf mosaic virus. The chlorotic mottling disease, characterized by yellow blotches which later coalesce on the leaves of the whole plant, caused most extensive damage because it not only reduced the photosynthetic leaf area but also produced distortions and severe plant stunting. Maize lethal necrosis disease mlnd develops from synergistic coinfection by sugarcane mosaic virus and maize chlorotic mottle virus. Maize chlorotic mottle virus exhibits low divergence between. First report of maize chlorotic mottle virus and maize lethal.
Pdf maize lethal necrosis disease mlnd develops from synergistic coinfection by sugarcane mosaic virus and maize chlorotic. To inform resistance strategies, we characterized the translation initiation mechanism of mcmv. This publication describe maize chlorotic mottle, the pathogen, vectors and virus transmission, and the disease epidemiology and symptoms. Maize chlorotic mottle virus mcmv, genus machlomovirus and potyviruses. Data sheet on maize lethal necrosis mln disease dsmz. Mln is caused by the synergistic interaction between sugarcane mosaic virus scmv and maize chlorotic mottle virus mcmv. We obtained maize chlorotic mottle virus genome sequences from across east africa and for the first time from ecuador and hawaii, and. When maize plants are coinfected with mcmv and scmv, maize dwarf mosaic virus mdmv, or. The maize lethal necrosis disease mlnd caused by synergistic interaction of maize chlorotic mottle virus and sugarcane mosaic virus, and has emerged as a serious threat to maize production in eastern africa since 2011.
Get a printable copy pdf file of the complete article 1. The complete nucleotide sequence of the maize chlorotic mottle. However, mcmv has never been found to infect sugarcane saccharum officinarum l. Genomewide association and genomic prediction of resistance. Crop science abstract maize responses to a severe isolate. It suggests integrated practices for successful management of maize cholorotic mottle and identifies areas of future research needed to gain a fuller understanding of maize chlorotic mottle. General information about maize chlorotic mottle virus mcmv00 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience.
We do not use these to store personal information about you. The disease caused severe economic losses in the midwest and southern united states during the 1960s to 1970s. The streaks then coalesce to form chlorotic mottling, which is then followed by leaf necrosis, epinasty. Pdf visual detection of maize chlorotic mottle virus. Ecological aspects of cereal chlorotic mottle virus. Soybean chlorotic mottle virus is easily distingushed from peanut chlorotic leaf streak caulimovirus d. How the pathogen survives and the primary source of inoculum is unknown. Our catalog 2020 can now be downloaded as pdf file. Machlomovirus is a genus of plant viruses, in the family tombusviridae. Maize chlorotic mottle virus mcmv mcmv is the only member of the genus machlomovirus family tombusviridae. Coat protein gene maize chlorotic mottle virus techne qpcr test 150 tests for general laboratory and research use only quantification of maize chlorotic mottle. Transgenic maize plants expressing mdmv strain b cp were resistant to mixed infections of mdmv and maize chlorotic mottle virus murry et al.
Concentration of maize chlorotic mottle virus increased in mixed infections with maize dwarf mosaic virus, strain b. Domestic regulation can be put in place to prevent the movement of maize products from affected areas. Seed inspectors can check for maize lethal necrosis disease mlnd in seed farms. This species also adults of both species overwinter and appear in the field. Mcmv is one of the most important plant pathogenic viruses and can induce various symptoms such as mild mosaic and lead crop losses of 10 to 15% in natural field infections and losses of up to 59% in experimental maize plots. Maize chlorotic mottle virus has been rapidly spreading around the globe over the past decade. Grind 4 replicates of 100 corn seeds, per sample and add 100 ml of general extraction buffer. Mcmv can be transmitted by different insects such as beetles or thrips. On the basis of significant amino acid sequence homology in the viral polymerase, the machlomovirus genus has been placed within the family tombusviridae. Clematis chlorotic mottle virus clcmv is a previously undescribed virus associated with symptoms of yellow mottling and veining, chlorotic ring spots, line pattern mosaics, and flower distortion and discoloration on ornamental clematis. Maize chlorotic mottle virus causes corn lethal necrosis disease, and can be transmitted via infected maize seeds. Seed maize produced in commercial nurseries in hawaii was harvested from symptomatic plants naturally infected by mcmv. Maize chlorotic mottle virus how is maize chlorotic mottle.
Notes on taxonomy and nomenclature top of page in 1963, a new maize disease was recognized in iowa, usa janson and ellett, 1963, and soon after was shown to be caused by a new virus named maize dwarf mosaic virus mdmv janson et al. First report of maize chlorotic mottle virus and maize lethal necrosis on maize in ethiopia. It has been reported in the major maize belts of uasin gishu, transnzoia, bomet, narok and nandi counties. Maize chlorotic mottle virus mcmv combines with a potyvirus in maize lethal necrosis disease mlnd, an emerging disease worldwide that often causes catastrophic yield loss. This publication describe maize chlorotic mottle, the pathogen, vectors and virus transmission, and the. The viral genome has four long open reading frames orfs which could encode polypeptides of 31. Plant viruses online maize chlorotic mottle machlomovirus. We report that, like other tombusvirids, mcmv rna contains a capindependent translation element cite in its 3. Apr 20, 2017 detection and characterization of maize chlorotic mottle virus and sugarcanemosaic virus associated with maize lethal necrosis disease in ethiopia. Similar to its brother virus, cowpea mosaic virus cpmv, ccmv is produced in high yield in plants. Mcmv serotypes show no significant differences in symptoms or pathogenicity. In order to determine the ability of western flower thrips frankliniella occidentalis.
Agdia positive control for maize chlorotic mottle virus mcmv. The potential geographical distribution of maize chloroticmottle virus in china basing on climex the 3level multiindex model for economic. Maize chlorotic mottle virus mcmv00reporting articles. Brome mosaic, cocksfoot mild mottle, cocksfoot mottle, cynosurus mottle, holcus transitory mottle, maize chlorotic dwarf, maize rayado fino, maize streak, maize stripe, maize white line, molinia streak, panicum mosaic, phleum mottle, rice yellow mottle. Symptoms are fine chlorotic yellow streaks developing in the youngest leaves about 10 days after inoculation. Dissecting the mode of maize chlorotic mottle virus. Pathogen maize chlorotic mottle virus is the only species in the genus machlomovirus family tombusviridae. The virus is also transmitted in a semi persistent manner by six different species of chrysomelid beetles. Nov 05, 2019 the complete sequence of soybean chlorotic mottle virus dna and the identification of a novel promoter. Maize chlorotic mottle virus mcmv, the only member of the genus machlomovirus in the family tombusviridae, can infect various crops and lead to typical mild mosaic symptoms 28. On the basis of significant amino acid sequence homology in the viral polymerase, the machlomovirus genus has. General information about cereal chlorotic mottle virus ccmov0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Mecanical and seed transmission has also been demonstrated 1.
In general, resistance was achieved by expressing the virus replicase andor cp gene in the transgenic plants, delivering the dna by bombarding actively growing embryogenic calli with dnacoated particles. Maize chlorotic mottle virus mcmv is one of the co. In nebraska greenhouse studies, the seeds were planted and 14 dayold seedlings were assayed for mcmv. The double infection of the two viruses gives rise to what. Maize chlorotic mottle virus is a unique member of the family tombusviridae.
This positive control is intended for use in elisa test formats. Or, place 4 replicates of 100 corn seeds into 100ml of general extraction buffer. Elisa, diseased maize plant samples reacted positively with the antiserum of maize chlorotic mottle virus mcmv. Isometric virus particles 30 nm in diameter were found in infected maize leaf tissues.
Pdf first report of maize chlorotic mottle virus and. Although major yield losses in field dent corn due to mcdv have not been. Experimentally, it has been shown that ccmv particles swell in the absence of divalent cations when the ph is raised from 5 to 7. Response of selected maize inbred germplasm to maize. By using mutated versions of mcmv lacking one or two expressible orfs in maize protoplast and plant inoculations it was shown that five of the proteins have functions similar to the homologous proteins in related tombusvirid genera. Traditional diagnostics applied on samples with typical field symptoms of mln have often given negative results using elisa or pcr for mcmv and sugarcane. The results of incidence ratings to assess the host response of maize zea mays l. Response of selected maize inbred germplasm to maize lethal necrosis disease and its causative viruses sugarcane mosaic virus and. Continuing to use this website means you agree to our use of cookies. Complete nucleotide sequence of a maize chlorotic mottle. Pdf transcripts of a maize chlorotic mottle virus cdna. Seed transmission was found by elisa and confirmed by symptomatology and mechanical transmission in 17 of 42 000 plants from 25 seed lots submitted by. Kay scheets publications oklahoma state university. Machlomovirus is a plant virus with icosahedral virions of 30 nm in diameter containing a singlestranded positivesense rna of.
Imported maize seeds were planted in a quarantine greenhouse and two seedlings with chlorotic mottle symptoms were observed. Jun 05, 2006 electrostatic properties of cowpea chlorotic mottle virus ccmv and cucumber mosaic virus cmv were investigated using numerical solutions to the poissonboltzmann equation. Maize chlorotic mottle virus mcmv is a small, plussense single stranded rna virus of the genus machlomo virus, family tombusviridae. Detection and identification of maize chlorotic mottle virus. Notes on taxonomy and nomenclature top of page maize chlorotic mottle virus mcmv is the type and only species of the machlomovirus genus lommel, 1995. Monoclonal antibodybased serological methods for maize. Characterising maize viruses associated with maize lethal. Complete nucleotide sequence of clematis chlorotic mottle. This negative control is intended for use in elisa test formats. Efficiency of aphid and thrips vectors in transmission of. A plant health inspectorate organization can test for maize chlorotic mottle virus mcmv in all seed coming into the country including the material for breeding. Top of page maize chlorotic mottle virus mcmv is the type and only species of the machlomovirus genus lommel, 1995. Cereal chlorotic mottle virus ccmov0overview eppo global.
In the natural host, viral particles can be produced at 12 mg per gram of. Maize lethal necrosis mln caused by the coinfection of two viruses, maize chlorotic mottle virus mcmv belonging to the tombusviridae group and any member virus from the potyviridae group mostly sugarcane mosaic virus scmv is one of the most important maize diseases in sub. Maize chlorotic mottle virus is spread by thrips, frankliniella williamsi and f. Maize chlorotic mottle an overview sciencedirect topics. Transmission of maize chlorotic mottle virus by chrysomelid. Detection and characterization of maize chlorotic mottle. Request pdf realtime taqman rtpcr for detection of maize chlorotic mottle virus in maize seeds maize chlorotic mottle virus mcmv causes corn lethal necrosis disease, and can be transmitted. The interactions of maize chlorotic mottle virus with potyviridae viruses causes an aggressive. The experimental host range of maize chlorotic mottle virus mcmv is restricted to the gramineae poaceae family with maize as a natural host. Coat protein gene maize chlorotic mottle virus pcr max ltd tm qpcr test 150 tests for general laboratory and research use only quantification of maize chlorotic. A maize lethal necrosis disease was observed in yunnan province of china. Maize chlorotic mottle virus atcc pv262 designation. The complete nucleotide sequence of the maize chlorotic mottle virus mcmv genome has been determined to be 4437 nucleotides.
The complete nucleotide sequence of the maize chlorotic mottle virus genome. Further characterization of maize chlorotic mottle virus. Maize chlorotic mottle virus mcmv is the type species of the genus machlomovirus. This disease was controlled for several years on kauai by destroying infected crops, but it has since. Recent activity seeking to limit spread of the disease is reliant on effective diagnostics. The rna of maize chlorotic mottle virus the essential. Information on the diversity and transmission of those viruses are limited in ethiopia.
Item 34 maize chlorotic mottle virus item 35 pea earlybrowning virus item 36 tomato brown rugose fruit virus item 37 tomato leaf curl new delhi virus item 38 zucchini green mottle mosaic virus 2 regioncountries of distribution of the following seven quarantine pests will be updated. Rapid and sensitive detection of maize chlorotic mottle. The maize lethal necrosis disease mlnd is a result of a combination of two viruses, the maize chlorotic mottle virus mcmov and any of the cereal viruses in the potyviridae group, like the sugarcane mosaic virus scmv, wheat streak mosaic virus wsmv or maize dwarf mosaic virus mdmv. This has not only threatened regional trade, but also seed industry. Cowpea chlorotic mottle virus, known by the abbreviation ccmv, is a virus that specifically infects the cowpea plant, or blackeyed pea. Maize chlorotic mottle virus mcmv now poses a significant threat to temperatezone seed corn crops and some of the locally developed tropical corn varieties. Mcmv is the only member of the genus machlomovirus family tombusviridae. The coinfecting viruses act synergistically to result in frequent plant death or severely reduce or negligible. Maize chlorotic mottle virus is easily differentiated from other viruses commonly infecting maize in the western hemisphere maize chlorotic dwarf virus, maize mosaic virus, maize rayado fino virus, maize stripe virusand maize white line mosaic virus, because none of these is mechanically transmissible. Maize lethal necrosis mln is a disease of maize caused by coinfection of maize with maize chlorotic mottle virus mcmv and one of several viruses from the potyviridae, such as sugarcane mosaic virus, maize dwarf mosaic virus, johnsongrass mosaic virus or wheat streak mosaic virus.
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